What is a vacuum manifold used for in the laboratory?

A vacuum manifold uses negative pressure to accelerate liquid transfer through packed columns, filter membranes, or separation media. Common applications include solid phase extraction (SPE), sample filtration, protein precipitation, and sample concentration for analytical workflows.

Can vacuum manifolds work with 96-well microplates?

Yes, microplate vacuum manifolds are designed to accommodate standard SBS-format 96-well filter plates and collection plates. They process all 96 wells simultaneously, dramatically increasing sample preparation throughput compared to individual cartridge methods.

What is the difference between SPE and filtration manifolds?

SPE manifolds are designed for solid phase extraction using packed sorbent cartridges or plates to selectively retain and elute target analytes. Filtration manifolds use membrane filters to clarify samples by removing particulates, cells, or precipitated proteins without sorbent chemistry.

What vacuum pressure should I use with a manifold?

Most vacuum manifold applications perform optimally between 5 and 15 inches of mercury (12 to 38 kPa). Excessive vacuum can damage membranes or cause sample foaming, while insufficient vacuum slows processing. Use a vacuum regulator for precise control.

How do I maintain a vacuum manifold?

After each use, rinse the manifold chamber and tubing with appropriate solvents. Regularly inspect gaskets and seals for wear. Calibrate vacuum regulators periodically and ensure proper filtration of drawn air to protect the vacuum pump from contamination.

Vacuum Manifolds | Arctic White

Acrylic Vacuum Manifold for 96 Well Deep Capture Plates

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